CHEM462/562

Spectrometer instructions

Part 1: Determination of the proton 90 degree pulse:

Excel spreadsheet for Pulsewidth increment: 2 microseconds

Excel spreadsheet for Pulsewidth increment: 5 microseconds

Formula used in Excel: I = I₀ * [sin (PW * pi / PW₁₈₀) * exp (- PW / P_decay)]

with:		PW	= pulse width (in microseconds)
		pi / PW180	= conversion factor: microseconds -> radians
		Pdecay	= decay of pulse power for long pulses

Part 2: T₁ relaxation: Use I = A - I₀ * exp (- D2 / T₁)

Part 3: T₂ relaxation: Use I = I₀ * exp (D2 / T₂)           

Part 4: Nuclear Overhauser Enhancement:            
           NOE / NOESY data for Cellobiose Octaacetate